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In this work we consider optical flow on evolving Riemannian 2-manifolds which can be parametrised from the 2-sphere. Our main motivation is to estimate cell motion in time-lapse volumetric microscopy images depicting fluorescently labelled cells of a live zebrafish embryo. We exploit the fact that the recorded cells float on the surface of the embryo and allow for the extraction of an image sequence together with a sphere-like surface. We solve the resulting variational problem by means of a Galerkin method based on vector spherical harmonics and present numerical results computed from the aforementioned microscopy data.

We study Tikhonov regularization for solving ill-posed operator equations where the solutions are functions defined on surfaces. One contribution of this paper is an error analysis of Tikhonov regularization which takes into account perturbations of the surfaces, in particular when the surfaces are approximated by spline surfaces. Another contribution is that we highlight the analysis of regularization for functions with range in vector bundles over surfaces. We also present some practical applications, such as an inverse problem of gravimetry and an imaging problem for denoising vector fields on surfaces, and show the numerical verification.

In this paper we present a decomposition algorithm for computation of the spatial-temporal optical flow of a dynamic image sequence. We consider several applications, such as the extraction of temporal motion features and motion detection in dynamic sequences under varying illumination conditions, such as they appear for instance in psychological flickering experiments. For the numerical implementation we are solving an **integro-differential** equation by a fixed point iteration. For comparison purposes we use a standard time dependent optical flow algorithm, which in contrast to our method, constitutes in solving a spatial-temporal **differential** equation.

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